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. 2018 Jul 4;9:1356. doi: 10.3389/fimmu.2018.01356

Figure 3.

Figure 3

Acute antibody-mediated rejection (AMR) established by performing kidney transplant at early stage after skin priming was concomitant with a high ratio of graft-infiltrating macrophages. Balb/c recipient mice were primed with C3H donor skin grafts for 4 days prior to receiving the C3H kidney transplant to establish the AMR model. On day 5 after kidney transplantation, the phenotypes of CD45+ immune cells infiltrated in the kidney graft were determined by flow cytometry. (A) Representative dot plots of CD11b+F4/80+ macrophages, CD8+ T cells, CD49b+ NK cells, CD4+ T cells, and CD220+ B cells. (B) Macrophages form the most prominent population in the kidney graft, followed by CD8+ T cells, NK cells, CD4+ T cells, and B cell subsets. The results represent the mean ± SD of at least four independent samples.