Table 6.
Stable QTLs for alfalfa WH identified in an F1 (3010 × CW 1010) pseudo-testcross population based on phenotypic data assessed in three consecutive winters at two locations.
| Parent | QTL code | Chr. | Year/Location | Peak marker | Peak LOD | R2 | Allele dir. | LSI (cM) | Flanking markers |
|---|---|---|---|---|---|---|---|---|---|
| 3010 | wh1 | 1A | (λ2), λ4, ϕ3 | TP995 | 7.1 | 0.13 | (+) | 90.8–93.2 | TP995–TP6492 |
| 3010 | wh2 | 7A | (λ3), ψ1, ϕ1 | TP24733 | 7.1 | 0.12 | (+) | 37.5–39.0 | TP55743–TP34483 |
| 3010 | wh3 | 1A | (λ2), ϕ3 | TP65855 | 5.4 | 0.11 | (+) | 98.2–104.5 | TP65855–TP86274 |
| 3010 | wh4 | 8A | (ψ2), ϕ2 | TP10810 | 5.1 | 0.09 | (−) | 74.1–74.8 | TP58070–TP10810 |
| 3010 | wh5 | 3A | (ψ3), ϕ3 | TP71671 | 5.1 | 0.09 | (+) | 47.6–50.1 | TP52425–TP67563 |
| 3010 | wh6 | 1C | (λ1), ψ1, ϕ1 | TP37162 | 4.1 | 0.07 | (+) | 96.3–99 | TP37162–TP57104 |
| 3010 | wh7 | 7A | (λ1), λ3, ϕ1 | TP58371 | 3.2 | 0.07 | (+) | 26.4–29.3 | TP58371–TP34795 |
| 3010 | wh8 | 4C | (λ2), ψ1, ϕ1 | TP2323 | 3.5 | 0.06 | (+) | 27.1–31.1 | TP6532–TP4218 |
| CW 1010 | ws1 | 7C | λ2, (λ3), ψ1 | TP54614 | 9.8 | 0.14 | (−) | 48.4–51.4 | TP38417–TP54614 |
| CW 1010 | ws2 | 8D | (λ3), ψ3, ϕ3 | TP52817 | 7.5 | 0.10 | (−) | 40.8–43.6 | TP52817–TP46951 |
| CW 1010 | ws3 | 7A | (ψ3), ϕ2 | TP71946 | 5.7 | 0.10 | (+) | 8.6–17.6 | TP78230–TP71946 |
| CW 1010 | ws4 | 7A | ψ2, ϕ2 | TP81779 | 5.3 | 0.10 | (+) | 31–32.1 | TP16325–TP70376 |
| CW 1010 | ws5 | 8D | (λ2), ϕ3 | TP2543 | 5.2 | 0.08 | (−) | 44.6–45.8 | TP2543–TP6748 |
| CW 1010 | ws6 | 7B | (λ3), ψ1, ϕ1 | TP87913 | 5.6 | 0.07 | (−) | 35.9–37 | TP87913–TP85708 |
| CW 1010 | ws7 | 7B | (λ3), ψ1, ϕ3 | MRG_41805356 | 4.9 | 0.07 | (−) | 24.5–25.3 | TP49165–TP74211 |
| CW 1010 | ws8 | 8D | (λ1), λ2 | TP8426 | 3.3 | 0.06 | (−) | 58.6–63.1 | TP69982–MRG_7512818 |
| CW 1010 | ws9 | 1A | (λ3), ϕ3 | TP40020 | 3.4 | 0.04 | (+) | 47.2–48 | TP60690–TP40020 |
Eight QTLs from 3010 to 9 QTLs from CW 1010 were mapped on respective genetic linkage maps using phenotypic datasets of more than one environment and/or year. The symbol with bracket in the column “year/location” indicates the source dataset from which the other parameters in the same row were generated.
JPC environment winter hardiness data for the period: λ1 = 2015; λ2 = 2016; λ3 = 2017.
BVL environment winter hardiness data for the period: ψ1 = 2015; ψ2 = 2016; ψ3 = 2017.
Across environment winter hardiness data for the period: ϕ1 = 2015; ϕ2 = 2016; ϕ3 = 2017.