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. 2018 Jul 5;71(1):56–72.e4. doi: 10.1016/j.molcel.2018.06.003

Figure 2.

Figure 2

H3K27Ac Levels Do Not Immediately Correlate with NuRD-Induced Gene Expression Changes

(A) H3K27Ac ChIP-seq data normalized to H3 ChIP-seq at indicated time points of tamoxifen addition are plotted across a metagene for genes showing significant reduction in mRNA levels within 8 hr (“repressed early”) or only after 24–48 hr (“repressed late”). Data are plotted as mean ± 95% confidence intervals (N = 3 biological replicates).

(B) As in (A) but for genes showing significant increases early (≤8 hr) or late (24–48 hr).

(C) ChIP-qPCR for H3K27Ac plotted relative to H3 ChIP across the Ppp2r2c and Htra1 transcription start sites for 0, 24, and 48 hr following tamoxifen addition. Mean ± SEM; N ≥ 3 biological replicates. (Below) Data at the peak of enrichment from the panels above are displayed across the time course of tamoxifen exposure. Mean ± SEM (∗∗p < 0.01 and ∗p < 0.05 relative to 0 hr). N = 2–5 biological replicates.

(D) ChIP-seq for H3K27Ac normalized to H3 ChIP for each time point of Mbd3 induction centered at peaks of NuRD binding ± 2 Kb, classified into active and inactive enhancers, promoters, or other sequences. Mean signal for each category is plotted across the top.