Figure 10.

Entamoeba histolytica does not induce Reactive Oxygen Species (ROS) formation. Human neutrophils were previously incubated with the ROS-sensitive fluorescent compounds (A) dihydrorhodamine 123 (15 μM), or (B) dihydroethidium (15 μM). Next, neutrophils were not stimulated (—), or were stimulated with 20 nM phorbol 12-myristate 13-acetate (PMA), or with E. histolytica trophozoites. After 1 h, cells were analyzed by flow cytometry (A) or by fluorescence microscopy (B). Data are representative of three independent experiments. Bar is 100 μm.