Figure 12.

Entamoeba histolytica induce activation of NF-κB. Human neutrophils were stimulated with E. histolytica trophozoites for various periods of time, and then cell lysates were prepared. Neutrophils were (A) left untreated, or previously treated with (B) the NF-κB inhibitor BAY117082 (5 μM), or with (C) the MEK inhibitor UO126 (75 μM). Proteins were resolved by SDS-PAGE, and then Western blotted for phosphorylated-NF-κB (p-NF-κB) (upper panel) or for total NF-κB (lower panels) to show equal loading of proteins. Plots on the right show densitometric analysis for the ratio of p-NF-κB/NF-κB. Data are mean ± SEM of three experiments.