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. 2018 Jul 4;8:226. doi: 10.3389/fcimb.2018.00226

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Copyright © 2018 Fonseca, Díaz-Godínez, Mora, Alemán, Uribe-Querol, Carrero and Rosales.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neutrophils in touch with Entamoeba histolytica release NETs. (A) Human neutrophils were stimulated with E. histolytica in the presence of SYTOX® Green. Live cell images were captured at different times with a fluorescence inverted microscope. External DNA fluorescence appears bright white in the pictures. Scale bar is 50 μm. (B) NETs formation was induced only in neutrophils that were in direct contact with E. histolytica trophozoites (orange arrows). The NETs were produced around the amoebas and progressively covered the parasites. Neutrophils that were not in contact with amoebas (white arrow heads) did not release DNA fibers and never became SYTOX® Green-positive. Scale bar is 25 μm.