Figure 6.

Entamoeba histolytica trophozoites did not change surface expression of receptor FcγRIIIb (CD16b). Human neutrophils were not stimulated (solid line) or were stimulated with E. histolytica trophozoites (dashed line) for 30 min at 37°C. Then, cells were fluorescence-stained with secondary antibody only (thin line), or with monoclonal antibody (mAb) 3G8 or with mAb CLB-gran1, both specific against FcγRIIIb, as described in section Materials and Methods. Fluorescence intensity was then analyzed by flow cytometry. Histograms are representative of three independent determinations.