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. 2018 Jul 4;8:226. doi: 10.3389/fcimb.2018.00226

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Copyright © 2018 Fonseca, Díaz-Godínez, Mora, Alemán, Uribe-Querol, Carrero and Rosales.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The NADPH-oxidase inhibitor DPI reduced Entamoeba histolytica-induced NETs formation. Human neutrophils were not stimulated (—), or were stimulated with 20 nM phorbol 12-myristate 13-acetate (PMA), or with E. histolytica trophozoites. Some neutrophils were previously treated with 10 μM diphenyleneiodonium (DPI), a NADPH-oxidase inhibitor (open symbols). The relative amount of NETs was estimated from SYTOX® Green fluorescence in relative fluorescent units (RFU) during 4 h after stimulation. Data are mean ± SEM of three experiments.