Supplemental Figure S2.

Coverage-related quality metrics. A: The coverage plots for all candidate alleles from the 30 German Bone Marrow Donor Center (DKMS) samples downsampled to 400 reads per sample are visualized for HLA-A, -B, and -C genes (gray lines). The mean coverage plots (black lines) are superimposed on individual plots. B: The CV of coverage depths at all positions across a candidate allele and its correlation with mean coverage depths of individual candidate alleles. A linear regression line is shown. C: Distribution of allele balance ratios, calculated as the total coverage of the minor allele divided by that of the dominant allele, across 30 DKMS samples. A horizontal reference line at the allele balance ratio of 1.00 is shown (dotted line).