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GSDMD regulates release of microparticulate active caspase 1. CE and MP fractions were isolated from CAS9 and CAS9/GSDM-knockout (CAS9/GSDMD-KO) cells in LPS (1 μg/ml) for 2 hours or were left untreated and analyzed for the presence of cleaved GSDMD and active p20 Casp1. Hsp90 was used as a loading control. Data and densitometric scan of immunoblot are representative of three experiments.
