Figure 1.

Quantification of follistatin-like 3 (FSTL3) via targeted proteomics in primary bronchial epithelial cells (BECs) derived from healthy donors and donors with asthma. (A) The peptide primary sequence, locations and numbers of cleavage ions, and representative images showing histograms of the indicated product ions quantified from two independent FSTL3 peptides in isolates from media samples obtained from healthy BECs compared with asthmatic BECs. (B) FSTL3 abundance was assessed by summing the peak area quantification of the product ions from two technical replicates from two independent experiments using healthy donors (n = 4) and donors with asthma (n = 4). FSTL3 abundance was decreased in media samples obtained from asthmatic BECs compared with healthy BECs. Significance was assessed using an unpaired Student’s t test (*P < 0.05).