Figure 6.

Selective ERβ agonism with diarylpropionitrile (DPN) dose dependently increases PHD2 and decreases HIF-1α and HIF-2α in cultured rat PAECs. (A) Representative Western blots showing the effects of DPN (1, 10, or 100 nM, or 1 μM) or vehicle (DMSO) on PHD2 protein expression during hypoxia (1% O₂; 48 h). (B–D) The protein expression of PHD2 (B), HIF-1α (C), and HIF-2α (D) was quantified with densitometry. Data represent the mean ± SEM from three independent experiments. *P < 0.05 versus vehicle control using one-way ANOVA with post hoc Dunnett’s test.