Table 3. Selection of allergen components for diagnostic work-up using specific IgE.
| Providers in Germany: | Euroimmun | Dr. Fooke Laboratorien | Omega Diagnostics | Siemens Healthcare | ThermoFisher Phadia | ||
|---|---|---|---|---|---|---|---|
| Test systems: | Euroline | Allerg- o-liq | Allergozyme IgE | Immulite 2000 |
a) ImmunoCAP
5a
b) ImmunoCAP ISAC 5b |
||
| Test concept and web information (cf legend) | Enzyme allergosorbent strip test1 | Reverse enzyme allergosorbent strip test2 | Enzyme allergosorbent strip test3 | Chemilumenescence enzyme immunoassay with liquid-phase allergens4 | a) Fluorescence enzyme allergosorbent test 5a
b) Multiple fluorescence enzyme allergosorbent test5b |
||
| Allergen sources | Species | Allergen components | |||||
| Tree pollen | Birch | Bet v 1 (major all.) | n (SPAC*) | r (RT301) | n (x901) | n (A89) | r (t215) |
| Bet v 2 (profilin) | n (SPAC) | r (RT302) | n (x907) | r (A127) | r (t216) | ||
| Ash/Olive | Ole e 1 (major all.) | – | – | n (A482) | r (t224) | ||
| Grass pollen | Timothy grass | Phl p 1 (major all.) | n (SPAC) | r (RG601) | n (x903) | – | r (g205) |
| Phl p 5 (major all.) | n (SPAC) | r (RG605) | n (x902) | – | r (g215) | ||
| Phl p 7 (polcalcin) | n (SPAC) | r (RG607) | – | – | r (g210) | ||
| Phl p 12 (profilin) | n (SPAC) | r (RG612) | – | – | r (g212) | ||
| u.v.a. | |||||||
| Herb pollen | Mugwort | Art v 1 (major all.) | – | r (RW601) | – | n (A753) | n (w231) |
| Ambrosia | Amb a 1 (major all.) | – | – | – | – | n (w230) | |
| Rosaceae | Appel | Mal d 1 (Bet v 1H) | – | r (RF491) | – | r (A464) | r (f434) |
| Peach | Pru p 3 (LTP) | – | – | – | n (A603) | r (f420) | |
| Tree nuts | Hazelnut | Cor a 1 (Bet v 1H) | – | r (RF171) | – | – | r (f428) |
| Cor a 9 (11S legumin) | – | – | – | – | r (f440) | ||
| Cor a 14 (2S albumin) | – | – | – | – | r (f439) | ||
| Leguminosae | Peanut | Ara h 2 (2S albumin) | – | r (RF132) | – | – | r (f432) |
r = recombinant component; n = natural component, purified from extracts, manufacturer-specific laboratory codes (in parentheses); Bet v 1H = Bet v 1-homologous PR10 protein; 2S albumin = storage protein; 11S legumin = storage protein; *SPAC = single purified allergen components, panel strip test (DP 3210-1601-1 E) with Bet v 4, Bet v 6,as well as extract of birch pollen and timothy grass pollen). Information on the test concepts: 1http://www.euroimmun.de/index.php?id=allergologie 2http://www.fooke-labs.de/downloads/flyer_allerg-o-liq_prinzip_email_2011-05.pdf 3http://static.omegadiagnostics.com.s3.amazonaws.com/product-downloads/ifu/Allergozyme_Spec._IgE_96T_-_36021000_-_DI02 0104_X_-_English.pdf 4http://healthcare.siemens.com/clinical-specialities/allergy/laboratorian-information 5ahttp://www.phadia.com/Laboratory/Allergy/Products/ImmunoCAP-Assays/ImmunoCAP-Specific-IgE/Test-Principle/ 5bhttp://www.phadia.com/Global/A%20Document%20Library/Allergy/Promotion%20Material/ImmunoCAP%20ISAC/ImmunoCAP- ISAC-Technical-brochure.pdf