Table 3. . Binding and optical properties of the near-IR fluorescence probes from scaffolds 4–13.

No.	Scaffold	Name	MW	λex/λem	Fold increase‡	Affinity (nM)	LogP	Φ (%)	Ref.
11	4	CRANAD-2	410.26	640/802†	70	38.0	3	0.6/40§	[49]
12	5	BAP-1	351.17	614/648¶	–	44.1	–	46.8¶	[70]
13	5	BAP-2	357.23	650/708¶	–	55	–	11.4¶	[71]
14	5	BAP-3	341.16	663/705¶	–	149	–	4.5¶	[71]
15	6	BAP-4	433.32	636/704¶	–	27	–	9.3¶	[71]
16	6	BAP-5	417.26	649/723¶	–	18	–	4.3¶	[71]
17	7	EUA-1	365.23	518/654¶	–	320	–	12#	[72]
18	7	EUA-2	483.36	597/667¶	–	230	–	7††	[72]
19	7	EUA-3	601.49	677/736¶	–	320	–	1‡‡	[72]
20	8	EUA-4	511.41	624/673¶	–	48.6	–	13‡‡	[72]
21	8	EUA-5	642.59	721/763¶	–	97	–	3‡‡	[72]
22	9	DANIR-2a	197.24	452/487†	1.5	1590	2.19	0§§	[73]
23	9	DANIR-2b	223.27	539/577†	2.9	35.8	2.79	0.66§§	[73]
24	9	DANIR-2c	249.31	597/665†	12.0	26.9	3.37	4.09§§	[73]
25	10	DANIR-3a	247.29	614/538¶¶	331	44.8	3.46	4.8/0.1§	[74]
26	10	DANIR-3b	273.33	682/615¶¶	716	8.8	4.05	29.9/0.3§	[74]
27	10	DANIR-3c	299.37	783/678¶¶	280	1.9	4.56	9.0/0.009§	[74]
28	10	DANIR-3d	325.41	828/716¶¶	5	–	5.10	3.7/0§	[74]
29	10	DANIR-3e	351.44	830/-¶¶	–	–	5.62	0.1/0§	[74]
30	11	DANIR-8a	291.35	616/550¶¶	57	–	–	10‡‡	[75]
31	11	DANIR-8b	317.38	683/619¶¶	321	38.5	3.69	22.3‡‡	[75]
32	11	DANIR-8c	343.42	798/678¶¶	629	14.5	4.30	7.1‡‡	[75]
33	11	DANIR-9a	321.37	613/573¶¶	3	–	–	7.5‡‡	[75]
34	11	DANIR-9b	347.41	685/642¶¶	17	197	3.04	15.7‡‡	[75]
35	11	DANIR-9c	373.45	787/673¶¶	194	19.9	3.73	9.3‡‡	[75]
36	12	Probe 5	227.26	400/532##	49.9	1590	2.55	–	[76]
37	12	Probe 6	255.31	440/554##	58.9	2300	3.24	–	[76]
38	13	ANCA-6	452.54	410/545##	7.7	1400	3.81	–	[77]
39	13	ANCA-14	467.56	385/530##	5.0	4600	2.79	–	[77]
40	13	ANCA-15	454.52	380/525##	5.1	13800	2.74	–	[77]
41	13	ANCA-16	497.58	430/540##	2.9	6700	2.53	–	[77]
42	13	ANCA-17	496.60	410/550##	8.4	1600	3.60	–	[77]
43	13	ANCA-18	434.53	–	–	–	–	–	[77]
44	13	ANCA-19	394.46	410/535##	6.6	1600	3.14	–	[77]

^†λex and λem were measured in PBS.

^‡Fold increase in fluorescence intensity upon binding with Aβ aggregates.

^§QYs were measured in PBS/Aβ40 aggregates respectively.

^¶Fluorescence excitation and emission and QY of compounds were determined with 10 μM of the compounds in CHCl₃.

^#In reference to rhodamine 6G in H₂O (λ_exc = 488 nm, Φ_F = 95%).

^††In reference to sulforhodamine in ethanol (λ_ex = 550 nm, Φ_F = 90%).

^‡‡In reference to crystal violet in methanol (λ_ex = 610 nm, Φ_F = 66%).

^§§QYs were determined using a calibrated integrating sphere. QYs were determined in dichloromethane.

^¶¶Determined in PBS (λ_em1) and upon binding with Aβ aggregates (λ_em2).

^##λex and λem were measured upon binding with Aβ aggregates.

λem: Maximum emission wavelength; λex: Maximum excitation wavelength; Aβ: β-amyloid; MW: Molecular weight; QY: Quantum yield.