Table 1.
Bacterial strains and plasmids used in study
| Description | References | |
|---|---|---|
| Strains | ||
| SENWT | Salmonella enterica serovar Enteritidis P125109 wild type; Smr | [18, 79] |
| SEN∆alr | SENalr mutant (SENalr::aphT); Smr Kmr | This study |
| SEN∆dadX | SENdadX mutant (SENdadX::aphT); Smr Kmr | |
| SEN∆3897 | SEN3897 mutant (SEN3897::aphT); Smr Kmr | |
| SEN ∆alr∆dadX | Double mutant of SENalr and SENdadX (SENalr::aphT, SENdadX::cat); Smr Kmr Cmr | |
| ∆ InvC | Salmonella Enteritidis TTSS-1 deficient strain (SENinvC::aphT); Smr Kmr | [18] |
| ∆ SsaV | Salmonella Enteritidis TTSS-2 deficient strain; ssaV mutant Smr | [80] |
| DH5α | Escherichia coli | |
| Codon Plus | Escherichia coli (DE3) Codon Plus | |
| Plasmids | ||
| pKD4 | Template plasmid; FRT-aphT-FRT (containing kanamycin resistance gene, Kmr) | [28] |
| pKD46 | Red recombinase expression plasmid (Ampr) | [28] |
| pKD3 | Template plasmid carrying chloramphenicol resistance gene flanked by FRT (Cmr) | [28] |
| pCJLA-GFP | GFP-plasmid (Ampr) | [81] |
| pET28a | His tag containing expression vector (Kmr) | |