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. 2018 Jul 11;23:30. doi: 10.1186/s11658-018-0096-y

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Western blot (WB) analyses of OCSC markers in DSPP-MMP20 silenced OSC2 cells (a). Normalization of ALDH1, ABCG2, BMI1, Podoplanin (PDPN), CD44, CD133 and LGR4 protein levels was with β-actin . Y- (vertical) axis of histograms represent expression level for each protein relative to β-actin (b). OSC2 = parent (untreated) cells; shC = control (scramble sequence) cells; shD = DSPP silenced cells; shM = MMP20 silenced cells; shDM = combined DSPP-MMP20 silenced cells. *p < 0.05 compared with ShC. Experiments were performed in triplicate