Fig. 2.

Effects of the LOX-dependent AA metabolites, 12-HETE, and 15-HETE, either alone or in combination, on DPDPE-mediated inhibition of PGE₂-stimulated cAMP accumulation. Primary cultures of peripheral sensory neurons were pretreated with 12-HETE (100 nM), 15-HETE (100 nM), or 12-HETE and 15-HETE combined for 45 minutes before incubation with BK (10 µM) for an additional 15 minutes to induce DOR functional competence. The amount of cAMP accumulated (15 minutes, 37°C) in response to PGE₂ (1 µM), with or without DPDPE (100 nM), was measured as described in Materials and Methods. Pretreatment with the HETEs had no effect on basal or PGE₂ -stimulated cAMP accumulation levels, which were 1.2 ± 0.6 pmol/well and 187% ± 34% above basal, respectively. Data represent mean ± S.E.M. of five experiments. *P < 0.05 versus vehicle (no DPDPE).