Fig. 6.

Inhibition of 12- and 15-LOX activity prolongs DOR functional competence for the inhibition of PGE2-stimulated cAMP accumulation in primary cultures of peripheral sensory neurons. Cells were incubated with or without the combination of 12-/15- LOX inhibitors (LOXi) luteolin (1 μM) and BAIC (1 μM) for 30 minutes before treatment with AA (50 µM) for 15 minutes. DPDPE (100 nM)-mediated inhibition of PGE₂-stimulated cAMP was measured immediately, or cells were washed and DPDPE-mediated responses were determined 15 minutes later (see time line). LOX inhibitors did not alter basal or PGE₂-stimulated cAMP accumulation, which were 0.6 ± 0.2 pmol/well and 205% ± 42% above basal, respectively. Data represent mean ± S.E.M. of four experiments * P < 0.05; **P < 0.01 compared with PGE₂ in the absence of DPDPE.