Fig. 7.

Inhibition of 12- and 15-LOX activity restores DOR system responsiveness for the reduction of PGE₂-evoked thermal allodynia after AA treatment. Rats received injections of AA (3 μg, 50 μl, intraplantarly) 30 minutes before injections of Veh or the LOX inhibitors (LOXi) LUT (3 μg) and BAIC (3 μg) in combination (50 μl, intraplantarly). Rats were then injected with BK (25 μg, intraplantarly) 15 minutes later to induce functional competence; 15 minutes after BK administration, DOR responsiveness was assessed by injecting PGE₂ (0.3 μg) along with DPDPE (20 μg) (50 µl, intraplantarly) and PWLs were measured before (baseline) and at 5-minute intervals after injection for 20 minutes. Data represent mean ± S.E.M. of the change from baseline PWL, six animals per group. *P < 0.05; **P < 0.01; ***P < 0.001 compared Veh-Veh.