The effect of LOX inhibitors on the duration of DOR system responsiveness to DPDPE on carrageenan-induced thermal allodynia. (A) Then, 15 minutes after intraplantar injection of carrageenan (CARRA, 500 µg), thermal allodynia was reduced completely by DPDPE (20 µg) Data represent mean ± S.E.M. of six rats/group. *P < 0.05; **P < 0.01 from CARRA-Veh. (B) Three hours after carrageenan injection, DPDPE no longer reduced thermal allodynia. After injection of the 12-/15-LOX inhibitors, reduction of carrageenan-induced thermal allodynia by DPDPE was restored. As shown in the time line, rats received intraplantar injections of Veh or a combination of the 12-LOX inhibitor, LUT (3 µg), and the 15-LOX inhibitor BAIC (3 µg) 2.5 hours after injection of carrageenan (500 µg). DPDPE (20 µg) was administered 30 minutes after LOX inhibitor pretreatment (i.e., responses to DPDPE were determined 3 hours after carrageenan administration). Data represent mean ± S.E.M. of six rats/group. *P < 0.05; **P < 0.01 from CARRA-Veh-DPDPE.