Figure 1.

Apelin improved AngII-induced senescence and suppressed ROS production. A – Cells were pre-treated with apelin (10^–8 M) for 1 h followed by exposure to AngII (10^–6 M) for an additional 24 h. Cell senescence was evaluated by SA-β-gal activity. Representative photo micrographs showed SA-β-gal-positive cells (blue) in HUVECs. B – P21 expression was detected by western blot. The bar graphs show the quantification of the indicated proteins. C – Cells were pre-treated with apelin (10^–8 M) for 1 h followed by exposure to AngII (10^–6 M) for an additional 24 h. Cellular ROS was evaluated by DCFH-DA. Representative photo micrographs show ROS-positive cells (green) in HUVECs

The values are expressed as means ± SD of at least 3 independent experiments or 5 random fields of vision.^*P < 0.05 vs. control; ^#P < 0.05 vs. AngII.