Figure 3.

Structural architecture of 3D spheroids. The SCC-040 and FaDu cells were maintained in adherent condition with standard medium (10% FBS) and next detached and placed as single cells on non-adherent plates in standard medium. The created spheroids were taken to make the formalin-fixed paraffin-embedded tissue sections (FFPET) and H&E staining as well as DAPI staining. A – cross section through the cells growing in 2D and 3D cultures of SCC-040 and FaDu cell lines, H&E staining (scale bars represent 20 μm and 50 μm, respectively); B – 3D structure stained with DAPI; blue – nuclei, pink – cells (scale bar represents 50 μm)