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. 2018 Jul 4;9:957. doi: 10.3389/fpls.2018.00957

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Copyright © 2018 Wu, Tian and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TRV-mediated virus-induced gene silencing (VIGS) of candidate SlARF genes in tomato e mutation leaves. SlARF8A and SlARF8B shared the same gene fragment in the VIGS vector. (A) Photographs of silencing tomato e mutation. (B) The candidate SlARF genes expression of injected with pTRV2-SlARF6A (a), pTRV2-SlARF8A (b), pTRV2-SlARF24 (c), pTRV2-SlARF6A/8A (d), pTRV2-SlARF6A/24 (e), pTRV2-SlARF8A/24 (f) cultures, respectively. (C) The SlARF genes expression of injected with pTRV2-SlARF6A/8A/24 culture. The expression of a specific SlARF was compared with the level in e mutant plant agroinfiltrated with empty vector (control sample). Standard errors (SE) are shown (n = 3).