FIG 2.

Soft ASW agar assays supplemented with dimethylsulfoniopropionate (DMSP), M. capitata mucus, or N-acetyl-d-glucosamine (NAG). (A) Average diameters of the swim circles of OCN008 and investigated mutants on soft ASW agar with 50 mM succinate (light gray bars) and soft ASW agar with 50 mM succinate and 10 mM DMSP (dark gray bars); (B) average diameters of the swim circles on soft ASW agar with 50 mM succinate (light gray bars) and soft ASW agar with 50 mM succinate and 1.0% (vol/vol) coral mucus (dark gray bars); (C) average diameters of the swim circles on soft ASW agar with 50 mM succinate (light gray bars) and soft ASW agar with 50 mM succinate and 10 mM NAG (dark gray bars). Error bars represent the standard deviations of the results of three independent trials, and an asterisk indicates strains with swim circles that are significantly larger on medium with a chemoattractant than on medium with succinate only (ANOVA, P < 0.05, n = 3 for each strain).