FIG 4.

A V. fischeri strain lacking hmp and aox has elevated NADH levels 6 h after treatment with nitric oxide. V. fischeri strains AKD915 (Δhmp) and AKD916 (Δhmp Δaox) were grown in mineral salts medium and treated with DPTA-NONOate. Samples were taken 6 h after treatment and assayed for NAD⁺ and NADH levels. AKD915 samples had an average of 1.25 (±0.098) μM NAD⁺ plus NADH and AKD916 samples had an average of 1.68 (±0.16) μM NAD⁺ plus NADH. Data shown are the averages from three experiments, each containing three independent replicates. Significance was measured using Student's t test. *, P < 0.02.