TABLE 2.
Fluorescence-based assays for reactive oxygen species production and membrane integrity in V. fischeri strains treated with nitric oxide
| Straina | Reactive oxygen species (APF) |
Membrane integrity (LIVE/DEAD) |
||
|---|---|---|---|---|
| Avg fluorescence (SE) | P valueb | Avg green/red fluorescence (SE) | P value | |
| AKD915 (Δhmp) | 635 (32) | 0.018 | 129 (11) | 0.026 |
| AKD916 (Δhmp Δaox) | 909 (33) | 88 (8) | ||
| AKD916 with pAKD601Baox | ||||
| Without IPTG | 782 (13) | 0.0007 | 91 (3) | 0.011 |
| With 0.1 mM IPTG | 646 (11) | 128 (7) | ||
a
Representative data shown for the comparison of AKD915 and AKD916 were collected in an experiment separate from that for the comparison of AKD916(pAKD601Baox) with and without IPTG.
b
P value calculated using Student's t test comparing AKD915 with AKD916 or comparing AKD916(pAKD601Baox) with and without IPTG.