TABLE 3.
Strains and plasmids
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| Vibrio fischeri | ||
| ES114 | Wild type | 13 |
| AKD915 | Δhmp (allele exchanged from pAKD915 into ES114) | This study |
| AKD916 | Δhmp Δaox (allele exchanged from pAKD780 into AKD915) | This study |
| Escherichia coli | ||
| DH5α | F− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR supE44 hsdR17 recA1 endA1 gyrA96 thi-1 relA | 31 |
| DH5αλpir | λpir derivative of DH5α | 32 |
| CC118λpir | Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA; lysogenized with λpir | 27 |
| Plasmids | ||
| pAKD915 | Δhmp allele, R6Kγ and ColE1 replication origins, RP4 oriT, Ermr Kanr | This study |
| pAKD780 | Δaox allele, R6Kγ and ColE1 replication origins, RP4 oriT, Ermr Kanr | 12 |
| pAKD601Baox | Plasmid containing lacIq and an IPTG-inducible promoter for controlled expression of Aox in V. fischeri, Kanr | 12 |
| pEVS104 | Conjugative helper plasmid, R6Kγ replication origin, Kanr | 27 |
a
Ermr, erythromycin resistance; Kanr, kanamycin resistance (aph).