TABLE 4.

Primers used in this study

Primer name	Primer sequence (5′→3′)	Use
hmpupF	GAGTTACCACGCTGAATCGCT	Forward primer for upstream fragment for hmp deletion construct
hmpupR	TTATAAAAATTAGTCGACGTTGAGCATATTGTTTACCTTTAAAGGTGTA	Reverse primer for upstream fragment for hmp deletion construct
hmpdownF	CAATATGCTCAACGTCGACTAATTTTTATAAAGTAGAAATGCAAAAACGCCCA	Forward primer for downstream fragment for hmp deletion construct
hmpdownR	CACGGTACTTACCCTTACGTAACATC	Reverse primer for downstream fragment for hmp deletion construct
hmpRepF	TACTACCTAGGACGCTTGCTTTGCTTTCGCT	Used with hmpTestR to screen for hmp deletion mutants
hmpTestR	TCTGTTACTGCTGATGGACGTAAACAT
msrAB F	CGCAAGAAGAGGGAACAGAA	Forward primer for qRT-PCR of VF_A0005
msrAB R	CCAACCAGTCCCGGATTTATAC	Reverse primer for qRT-PCR of VF_A0005
pspA F	CCAACAAACTGCACAGCATAG	Forward primer for qRT-PCR of VF_A0311
pspA R	CTCATCAATTCGGCGTTCATATT	Reverse primer for qRT-PCR of VF_A0311
argH F	ATTGGCTGATACCGTGACTTC	Forward primer for qRT-PCR of VF_2303
argH R	CCATATACACGTCCACACTTACC	Reverse primer for qRT-PCR of VF_2303
ftsZ F	GTGCGTGTCGTTGAAGTTATTC	Forward primer for qRT-PCR of VF_0905
ftsZ R	GGCTACCTACAACATCAGAGAAG	Reverse primer for qRT-PCR of VF_0905