FIG 5.
NtrC from V. cholerae allows growth under limiting nitrogen availability. (A) Images of spot colonies from E. coli and the ΔntrC strain harboring an empty plasmid (pBAD) or a complementation plasmid expressing ntrB-ntrC or ntrB-ntrCD56A from V. cholerae. Cells were grown on M9 agar supplemented with 100 mM l-serine as a nitrogen source and 0 or 0.1% arabinose (for induction) for 24 h at 37°C. (B) Images of spot colonies from V. cholerae and the ΔntrC strain harboring an empty plasmid (pBAD) or a complementation plasmid expressing ntrC or ntrCD56A. Cells were grown on M9 agar supplemented with 100 mM l-arginine as a nitrogen source and 0 or 0.1% arabinose for 24 h at 37°C.