TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or description | Reference or source(s) |
|---|---|---|
| Strains | ||
| E. coli | ||
| CC118λpir | Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 λpir | 53 |
| S17-1λpir | Tpr Smr recA thi pro (rK− mK+) RP4::2-Tc::Mu-Km Tn7 λpir | 54 |
| K-12 (BW28357) | lacIp4000(lacIq) rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1 | 55 |
| K-12ΔntrBC (BW30011) | lacIp4000(lacIq) rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1 Δ(ntrB-ntrC)1318 | 55 |
| V. cholerae | ||
| FY_VC_0001 | Vibrio cholerae O1 El Tor A1552; wild type; Rifr | 6 |
| FY_VC_0616 | PvpsL-lacZ; Rifr | 27 |
| FY_VC_2272 | ΔVC0665 (vpsR) | 4 |
| FY_VC_0192 | ΔVC1021 (luxO) | 47 |
| FY_VC_7998 | ΔVC1522 | 47 |
| FY_VC_8243 | ΔVC1926 (dctD-1) | 47 |
| FY_VC_6286 | ΔVC2135 (flrC) | 47 |
| FY_VC_6289 | ΔVC2749 (ntrC) | 47 |
| FY_VC_8245 | ΔVCA0142 (dctD-2) | 47 |
| FY_VC_2407 | ΔVC0665 mTn7-gfp Rifr Gmr | This study |
| FY_VC_8068 | ΔVC1021 mTn7-gfp Rifr Gmr | This study |
| FY_VC_8070 | ΔVC1522 mTn7-gfp Rifr Gmr | This study |
| FY_VC_8319 | ΔVC1926 mTn7-gfp Rifr Gmr | This study |
| FY_VC_8072 | ΔVC2135 mTn7-gfp Rifr Gmr | This study |
| FY_VC_7061 | ΔVC2749 mTn7-gfp Rifr Gmr | This study |
| FY_VC_8321 | ΔVCA0142 mTn7-gfp Rifr Gmr | This study |
| FY_VC_7058 | ΔVC2748 (ntrB) | This study |
| FY_VC_7057 | ΔntrC ΔvpsR | This study |
| FY_VC_0099 | ΔvpsT | 5 |
| FY_VC_7051 | ΔntrC ΔvpsT | This study |
| FY_VC_7936 | ΔVC2529 (rpoN) | This study |
| FY_VC_7304 | ΔntrC ΔrpoN | This study |
| FY_VC_0178 | ΔhapR | This study |
| FY_VC_7054 | ΔntrC ΔhapR | This study |
| FY_VC_2326 | Δcrp | 9 |
| FY_VC_7056 | ΔntrC Δcrp | This study |
| FY_VC_13619 | ΔrpoN ΔhapR | This study |
| FY_VC_13621 | ΔrpoN ΔntrC ΔhapR | This study |
| Plasmids | ||
| pGP704sacB28 | pGP704 derivative; mob-oriT sacB Apr | G. Schoolnik |
| pFY-1422 | pGP704-sacB28::ΔVC2748; Apr | This study |
| pBBRlux | luxCDABE-based promoter fusion vector; Cmr | 28 |
| pFY-0950 | pBBRlux vpsL promoter; Cmr | 56 |
| pFY-1326 | pBBRlux glnA promoter; Cmr | This study |
| pFY-0989 | pBBRlux vpsR promoter; Cmr | This study |
| pFY-0988 | pBBRlux vpsT promoter; Cmr | This study |
| pFY-1049 | pBBRlux hapR promoter; Cmr | This study |
| pBAD/myc-His-B | Arabinose-inducible expression vector with C-terminal myc epitope and six-His tags | Invitrogen |
| pFY-1648 | pBAD/myc-His-B::ntrBCVCa; Apr | This study |
| pFY-1351 | pBAD/myc-His-B::ntrC; Apr | This study |
| pFY-1349 | pBAD/myc-His-B::ntrCD56A; Apr | This study |
| pUX-BF13 | oriR6K helper plasmid; mob-oriT; provides the Tn7 transposition function in trans; Apr | 57 |
| pMCM11 | pGP704::mTn7-gfp; Gmr Apr | M. Miller and G. Schoolnik |
a
VC, V. cholerae.