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Overexpression Egr-1 by M61-Egr-1 transfection promoting EMT in NRK-52E cells. NRK-52E cells were transfected by M61-Egr-1 before 10 ng/ml TGF-β1 stimulation. (a–c) The mRNA (a) and protein (b, c) expressions of Egr-1. (d–f) The mRNA expressions of E-cadherin, α-SMA, and FN. (g–j) The protein expressions of E-cadherin, α-SMA, and FN. Values are shown as mean ± SD. n = 3 for each group. ∗p < 0.05, ∗∗p < 0.01, #p < 0.05, ##p < 0.01, &p < 0.05, and &&&p < 0.001 versus vector group.
