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Changes of absorption spectra of ICG cultured with or without cells and/or illumination. (a) Absorption spectra of PBS-based media containing 10 μg/mL ICG after a 24-hour incubation with or without cells cultured in the dark or under illumination. (b, c) Bright-field micrographs in cells cultured with or without 10 μg/mL ICG in the dark or under illumination.
