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. 2018 Jun 27;2018:4957573. doi: 10.1155/2018/4957573

Figure 5.

Figure 5

SIRT1 knockdown suppressed FP-induced changes in the expression of SIRT1, collagen I/III, MMP-2/9, TGF- β 1, and p-Smad2/3 in Ang II-treated CFs. (a) The SIRT1 level was significantly knocked down by siRNA. (b) Quantitative analysis of (a). (c) The SIRT1 level was lower in the siRNA-transfected CFs compared to the NC and untransfected CFs stimulated by Ang II. (d) Quantitative analysis of (c). (e) Representative Western blot images showing that SIRT1-siRNA antagonized the FP-induced decrease in the expression of collagen I/III, MMP-2/9, TGF-β1, and p-Smad2/3. GAPDH was used as an internal control. (f) Quantitative analysis of (e). Data are expressed as the mean ± SEM; n = 6 per group; ∗P < 0.05 versus the SIRT1 siRNA, Ang II+SIRT1 siRNA, or Ang II+NC+FP group; #P < 0.05 versus the Ang II+SIRT1 siRNA group. NC: the negative control group.