Table 1.
Alteration of inflammatory environment.
| Cytokine release | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| IL-1β | IL-2 | IL-4 | IL-5 | IL-6 | IL-10 | IL-12-p70 | IL-13 | IFN-gamma | TNF-alpha | ||
| T-cells | unstimulated | 3.64 ± 2.51 p = 1.000 |
0.01 ± 0.01 p = 1.000 |
0.08 ± 0.02 p < 0.001 |
0.00 ± 0.00 p < 0.001 |
0.26 ± 0.14 p < 0.001 |
0.01 ± 0.01 p < 0.001 |
0.04 ± 0.03 p < 0.001 |
0.00 ± 0.00 p < 0.001 |
0.00 ± 0.00 p < 0.001 |
0.02 ± 0.01 p < 0.001 |
| stimulated | 1.00 ± 0.00 | 1.00 ± 0.00 | 1.00 ± 0.00 | 1.00 ± 0.00 | 1.00 ± 0.00 | 1.00 ± 0.00 | 1.00 ± 0.00 | 1.00 ± 0.00 | 1.00 ± 0.00 | 1.00 ± 0.00 | |
| BM-MSC | unstimulated | 0.43 ± 0.06 p = 1.000 |
0.24 ± 0.17 p = 1.000 |
0.33 ± 0.26 p = 0.002 |
0.01 ± 0.00 p < 0.001 |
1.25 ± 0.08 p = 0.495 |
0.01 ± 0.00 p < 0.001 |
0.37 ± 0.04 p = 0.001 |
0.00 ± 0.00 p < 0.001 |
0.01 ± 0.00 p < 0.001 |
0.04 ± 0.00 p < 0.001 |
| stimulated | 2.74 ± 0.85 p = 1.000 |
27.24 ± 18.15 p = 0.070 |
0.33 ± 0.25 p = 0.002 |
0.01 ± 0.00 p < 0.001 |
1.25 ± 0.11 p = 0.476 |
0.01 ± 0.00 p < 0.001 |
0.40 ± 0.09 p = 0.002 |
0.00 ± 0.00 p < 0.001 |
0.01 ± 0.00 p < 0.001 |
0.05 ± 0.01 p < 0.001 |
|
| CMPC | unstimulated | 5.86 ± 2.34 p = 0.996 |
0.01 ± 0.00 p = 1.000 |
0.50 ± 0.12 p = 0.051 |
0.01 ± 0.00 p < 0.001 |
1.09 ± 0.03 p = 0.999 |
0.02 ± 0.01 p < 0.001 |
0.50 ± 0.19 p = 0.017 |
0.00 ± 0.00 p < 0.001 |
0.01 ± 0.00 p < 0.001 |
0.06 ± 0.01 p < 0.001 |
| stimulated | 36.49 ± 2.32 p = 0.005 |
1.03 ± 0.22 p = 1.000 |
0.61 ± 0.15 p = 0.233 |
0.01 ± 0.00 p < 0.001 |
1.09 ± 0.04 p = 0.999 |
0.03 ± 0.00 p < 0.001 |
0.55 ± 0.23 p = 0.047 |
0.00 ± 0.00 p < 0.001 |
0.01 ± 0.00 p < 0.001 |
0.07 ± 0.01 p < 0.001 |
|
| BM-MSC + T-cells | unstimulated | 0.51 ± 0.12 p = 1.000 |
0.22 ± 0.07 p = 1.000 |
0.21 ± 0.08 p < 0.001 |
0.00 ± 0.00 p < 0.001 |
1.26 ± 0.07 p = 0.123 |
0.01 ± 0.00 p < 0.001 |
0.17 ± 0.13 p < 0.001 |
0.00 ± 0.00 p < 0.001 |
0.01 ± 0.00 p < 0.001 |
0.05 ± 0.02 p < 0.001 |
| stimulated | 9.13 ± 2.93 p = 0.689 |
31.71 ± 10.07 p = 0.001 |
0.28 ± 0.10 p = 0.005 |
0.01 ± 0.00 p < 0.001 |
1.29 ± 0.07 p = 0.065 |
0.01 ± 0.00 p < 0.001 |
0.14 ± 0.03 p < 0.001 |
0.01 ± 0.00 p < 0.001 |
0.18 ± 0.04 p < 0.001 |
0.08 ± 0.01 p < 0.001 |
|
| CMPC + T-cells | unstimulated | 8.10 ± 1.84 p = 0.777 |
0.01 ± 0.00 p = 1.000 |
0.25 ± 0.03 p < 0.001 |
0.00 ± 0.00 p < 0.001 |
1.09 ± 0.02 p = 0.983 |
0.06 ± 0.02 p < 0.001 |
0.12 ± 0.04 p < 0.001 |
0.00 ± 0.00 p < 0.001 |
0.01 ± 0.00 p < 0.001 |
0.06 ± 0.01 p < 0.001 |
| stimulated | 52.41 ± 5.21 p < 0.001 |
1.17 ± 0.07 p = 1.000 |
0.44 ± 0.06 p = 0.005 |
0.01 ± 0.00 p < 0.001 |
1.07 ± 0.02 p = 0.997 |
0.01 ± 0.00 p < 0.001 |
0.15 ± 0.049 p < 0.001 |
0.00 ± 0.00 p < 0.001 |
0.16 ± 0.03 p < 0.001 |
0.09 ± 0.01 p < 0.001 |
|
Table showing the fold-increase or decrease in cytokine production compared to the stimulated T-cells. P-values are shown where applicable.
IL = Interleukin, IFN-gamma = interferon-gamma, TNF-alpha = tumor necrosis factor-alpha, MSC = mesenchymal stem cells, CMPC = cardiomyocyte progenitor cells.