Fig 2. Components of the Slit signaling family are conserved in G. bimaculatus.

A) The type, number, and order of protein domains was assessed by using Drosophila Slit, isoform F (AGB93549) and the putative G. bimaculatus Slit (TRINITY_DN152282_c0_g1_i2) as queries in a conserved domain search in NCBI. Both Drosophila and G. bimaculatus Slit had multiple leucine-rich repeats (red rectangles), though the number differed. Smaller red rectangles represent the C-terminal domain of a LRR. Drosophila Slit has five EGF domains (blue ovals), while the predicted G. bimaculatus Slit has only four. Both have a laminin G-like domain (diamond), and a C-terminal cysteine knot (black circle). B) An NCBI conserved domain search was used to identify the domains present in Drosophila Robo1, isoform C (AAM71113) and putative G. bimaculatus Robo1 (TRINITY_DN172482_c0_g4_i2), which included one or two IG domains (purple circular sectors) and an Ig I-set domain (pink). In addition, both predicated Robo1 proteins had three Fibronectin-type 3 domains (green rectangles). C) An NCBI conserved domain search was used to identify the domains present in Drosophila Robo2, isoform A (AAF51375) and Robo3, isoform B (AGB92403) and putative G. bimaculatus Robo2/3 (TRINITY_DN174950_c3_g2_i1). Both Drosophila Robos had several Ig domains predicted, but only Robo 2 showed specific hits in the conserved domain search (purple). Drosophila Robo3, isoform A possessed a single Ig I-set domain. These Robos had either three or two fibronectin-type 3 domains (green rectangles). The predicted G. bimaculatus Robo2/3 has characteristics that matched Robo 2 better (at least one Ig domain), though the overall sequence similarity was closer to Robo 3, isoform B. D) Alignment of Commisureless from G. bimaculatus (Gb putative Comm) and Drosophila melanogaster (Dm Comm3-E). Black shading indicates identical amino acids, gray shading indicates amino acids. The Drosophila comm domain is noted by a red bar above the sequence.