A) The type, number, and order of protein domains was assessed by using Drosophila Netrin A, isoform C (AHN59713) and the putative G. bimaculatus Netrin A (TRINITY_DN160784_c2_g1_i2) as queries in a conserved domain search. Drosophila Netrin A, isoform C possessed a laminin N-terminal domain (yellow rectangle), three laminin-type EGF-like domains (blue ovals), and a netrin-like domain (red rectangle). The angled lines on the G. bimaculatus protein representation indicate missing N-terminal sequence, but the remaining sequence matches that of Drosophila, with three laminin-type EGF-like domains (blue ovals), and a netrin-like domain (red rectangle). B) The type, number and order of protein domains in Drosophila Frazzled, isoform A (AAF58493) and the putative G. bimaculatus Frazzled (TRINITY_DN172587_c0_g1_i7) was assessed using a conserved domain search. Frazzled had at-least two Ig-like domains (purple) and 6 Fibronectin Type III domains. The predicted G. bimaculatus protein was similar, though some sequence was missing from the N-terminal end (angled lines).