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. 2018 Jul 11;13(7):e0200108. doi: 10.1371/journal.pone.0200108

Fig 1. Light field microscope system used for 3D phenotypic imaging.

Fig 1

Green and red ray bundles show how different lateral positions in the sample are focused onto different elements into the microlens array. Abbreviations: C–condenser lens; MO–microscope objective; piezo–piezoelectric nosepiece focusing stage; TL–tube lens; sCMOS–scientific CMOS camera. Inset shows (from left to right): A raw light field micrograph of a swimming C. elegans specimen; a perspective (pinhole) view of the object formed by extracting the same pixel from each microlens subimage; a focused view of the worm formed by summing the signal from all pixels within a microlens subimage. Pixels using to create each image are shown in red.