Skip to main content
. 2018 Jun 28;16(6):e2006459. doi: 10.1371/journal.pbio.2006459

Fig 1. A speed–fidelity trade-off in the poliovirus RdRp.

Fig 1

(A) Relative fitness of 3DG64S as measured by direct competition. The amount of each virus at each passage was compared to the input and expressed as the difference in the log10 ratio in RNA genomes for 3DG64S (open circles) relative to WT over time. The slope of the dotted lines are the relative fitness values, 0.78 ± 0.01, n = 3 replicates. (B) Cumulative distribution function of fitness values for SNVs of poliovirus as determined in [16]. “*” indicates the relative fitness (0.78) and percentile (64th) of 3DG64S. (C) Plaque size of clones from WT (n = 272; black) and 3DG64S (n = 220; grey) virus populations. Box plots show median, 25% and 75% quartiles, and 1.5× interquartile range. ***p ≤ 0.005; t test with Welch’s correction. (D) Single-cycle growth curve for WT (filled circles, black line) and 3DG64S (open circles, grey line) in HeLa. Data are mean ± standard deviation (n = 5 replicates). ***p < 0.005; unpaired t test comparing WT and 3DG64S separately for each time point. (E) Single-cycle growth curve for WT (filled circles, black line) and 3DG64S (open circles, grey line) in 3T3 cell line derived from MEFs of PVR transgenic mice. Data are mean ± standard deviation (n = 5 replicates). **p < 0.01; ***p < 0.005; unpaired t test comparing WT and 3DG64S separately for each time point. (F) Relative fitness of 3DG64S (open circles) as measured by competition assay (see panel A) in the presence of varying concentrations of ribavirin. Note that the baseline relative fitness of 3DG64S (y-intercept) is lower than the fitness reported in panel A because the assays were performed under different experimental conditions (see Methods). (G) Mutation rate in mutations per nucleotide per strand copied for WT (filled circles) and 3DG64S (open circles) in the presence of varying concentrations of ribavirin, as determined by Luria Delbruck fluctuation test. All plotted data can be found in S1 Data. HeLa, human epithelial cells; MEF, mouse embryonic fibroblast; PVR, poliovirus receptor; RdRp, RNA-dependent RNA polymerase; SNV, single-nucleotide variant; WT, wild-type.