Figure 8. Gcm impacts the development and the inflammatory response of hemocytes.
Relative expression levels of plasmatocyte and lamellocyte markers, Jak/Stat inhibitor and cytokines in hemocytes from stage 16 mutant embryos (srp(hemo)> RFP,gcm [Cattenoz et al., 2016]) or from third instar mutant larvae (gcm> gcm KD). The levels represented are relative to the levels in controls (srp(hemo)> RFP for the embryos and gcm> for the larvae) after normalization against housekeeping genes. The data are displayed as described in Figure 1f.
Figure 8—figure supplement 1. Gcm is not induced in circulating hemocytes nor in lymph glands of 3rd instar larvae upon wasp infestation.
Immunolabelling of hemocytes from third instar larvae gcm> GFP without (a) or after wasp infestation (b). (DAPI in blue, GFP in green and Phalloidin in gray). (c) Immunolabelling of the lymph gland from third instar larva gcm> GFP after wasp infestation. (DAPI in blue, GFP in green). (d) Bright-field image of the melanised tumor induced by wasp infestation in gcm> gtrace LacZ Wandering L3 shown in Figure 9e. Scale bar: 100 µm.