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. 2018 Jul 11;7:e34890. doi: 10.7554/eLife.34890

Key resources table.

Designation Source or reference Identifiers Additional information
Mouse anti-L4 Pr. I. Ando Mouse anti-L4 (1/30)
Rabbit anti-PH3 Upstate biotechnology #06–570 Rabbit anti-PH3 (1/1000)
Mouse anti-Hemese Pr. I. Ando Mouse anti-Hemese (1/30)
Chicken anti-GFP abcam #13970 Chicken anti-GFP (1/500)
Rabbit anti-Serpent Trebuchet, unpublished Rabbit anti-Serpent (1/1000)
Rabbit anti-Stat92E Dr. D. Montell Rabbit anti-Stat92E (1/100)
Rabbit anti-PPO1 Dr. WJ. Lee Rabbit anti-PPO1 (1/100)
Rabbit anti-RFP abcam #62341 Rabbit anti-RFP (1/500)
S2 cells ATCC Ref: CRL-1963 S2 ATCC
Effectene
Transfection Reagent		 Qiagen Effectene Transfection
Reagent
WT Bloomington #5905 w1118
hopTum-l Bloomington #8492 hopTum-l/FM7c Point mutation that
constitutively activates
the Jak/Stat pathway
HmlΔRFP Makhijani et al., 2011 HmlΔRFP
UAS-hopTum-l Harrison et al., 1995





UAS-hopTum-l/CyO,
twilacZ		 Reporter line for hop
Tum-l over-expression
Gcm> GFP Soustelle and Giangrande, 2007
gcmGal4,UAS-mCD8
GFP/CyO,Tb		 Driver specific to embryonic
 hemocytes and glia, gcm
 hypomorphic mutation
Gcm KD Bloomington #31519 UAS-gcmRNAi dsRNA reporter line
for gcm down-regulation
Gcm GOF Bernardoni et al., 1997




UAS-gcmF18A Reporter line for gcm
over-expression
Gcm26 Vincent et al., 1996



gcm26/CyOactinGFP Null gcm mutation
Upd2Δ Bloomington #55727 upd2Δ 4.7 kb deletion
Upd3Δ Bloomington #55728 upd3Δ Imprecise excision
Upd2 KD Bloomington #33988 UAS-upd2RNAi dsRNA reporter line
 for upd2 down-regulation
Upd3 KD Bloomington #32859 UAS-upd3RNAi dsRNA reporter line
 for upd3 down-regulation
Upd2 GOF Jiang et al., 2009 UAS-upd2/CyO Reporter line for upd2
 over-expression
Upd3 GOF Jiang et al., 2009 UAS-upd3/CyO Reporter line for upd3
 over-expression
Ptp61F KD Bloomington #32426 UAS-Ptp61FRNAi dsRNA reporter line for
Ptp61F down-regulation
Socs36E KD Bloomington #35036 UAS-Socs36ERNAi dsRNA reporter line for
Socs36E down-regulation
Socs44A KD Bloomington #42830 UAS-Socs44ARNAi dsRNA reporter line for
Socs44A down-regulation
Ptp61Fa GOF Müller et al., 2005




UAS-Ptp61Fa/CyO Reporter line to over-express
the cytoplasmic splicing
isoform
Ptp61Fc GOF Müller et al., 2005

UAS-Ptp61Fc/TM3 Reporter line to over-express
the nuclear splicing isoform
Gcm(hemo)>  Cattenoz et al., 2016

gcmGal4,UAS-mCD8GFP,
repoGal80/CyO		 gcm driver not expressed
 in glia, hypomorphic mutation
sn>  Zanet et al., 2012

snGal4 Singed driver, specific to
embryonic hemocytes
srp(hemo)>  Brückner et al., 2004

srp(hemo)Gal4 Serpent driver specific to
embryonic hemocytes
dot>  Bloomington #67608 DotGal4 Dorothy driver specifically
expressed in embryonic and
larval lymph gland
lz> GFP Bloomington #6314 lzGal4,UAS-mCD8GFP Lozenge driver expressed
in crystal cells
10xStat92E-GFP Bloomington #26198 10xStat92E-GFP Reporter line for STAT activity,
10 Stat92E binding sites
driving GFP expression
Gtrace Bloomington #28282 UAS-FLP;;Ubi-p63E
(FRT.STOP)Stinger		 This line allows the analysis
of lineage-traced expression
 of Gal4 drivers
Ubi>  Bloomington #32551 UbiGal4 Expresses GAL4 in all cells
RFP Bloomington #30556 UAS-RFP Inserted on the second
chromosome
Gtrace-LacZ Bloomington #6355 P{Act5C> polyA>
lacZ.nls1}3
Fiji Schindelin et al., 2012

Fiji
pPac-gcm Miller et al., 1998


pPac-gcm
repoGFP Laneve et al., 2013

4.3 kb repo-GFP
pUAS-hopTum-l Harrison et al., 1995


pUAS-hopTum-l
pUAS-gcmRNAi Vienna Drosophila
Resource Center
(VDRC) #dna1452		 pUAS-gcmRNAi