Table 1.

Primer sets used in the site-directed mutagenesis of the cDNA encoding human human SULT2A1

SULT2A1 Allozyme	Amino Acid Substitution	Mutagenic Primer Set
SULT2A1*2	Met57Thr	5′-gagattctctgcctgacgcactccaagggggat-3′ 5′-atccccttggagtgcgtcaggcagagaatctc-3′
SULT2A1*3	Thr90Ser	5′-acagcactcagtgaatcggagagtccacgttta-3′ 5′-taaacgtggactctccgattcactgagtgctgt-3′
SULT2A1*4	Leu159Val	5′-tgtcaaggaaccgtggtatatggtcatggttt-3′ 5′-aaacatgacccatataccacagttccttgaca-3′
SULT2A1*5	Glu186Val	5′-ttactgagttatgaggtgctgaaacaggacaca 3′ 5′-tgtgtcctgtttcagcacctcataactcagtaa-3′
SULT2A1*6	Met57Thr/Glu186Val	*2 + *5
SULT2A1*7	Ala63Pro	5′-ccaagggggatcccaagtggatc-3′ 5′-gatcacttgggatcccccttgg-3′
SULT2A1*8	Lys227Glu	5′-tgaaagaaaacgagatgtccaat-3′ 5′-attggacatctcgttttctttca-3′
SULT2A1*9	Ala261Thr	5′-acttcacagtgacccaagctgaa-3′ 5′-ttcagcttgggtcactgtgaagt-3′
SULT2A1*10	Ala63Pro/Ala261Thr	*7 + *9

SULT, cytosolic sulfotransferase.