Table 2.

Kinetic constants of the sulfation of DHEA and tibolone by human SULT2A1 allozymes

DHEA as Substrate
	Vmax (nmol/min/mg)	Km (μM)	Vmax/Km
SULT2A1*1	83.2 ± 4.6	10.9 ± 1.3	7.63
SULT2A1*2	75.5 ± 4.6	9.96 ± 1.4	7.58
SULT2A1*3	76.8 ± 3.7	11.9 ± 1.2	6.45
SULT2A1*4	93.6 ± 6.7	11.1 ± 1.7	8.46
SULT2A1*5	84.6 ± 5.3	11.8 ± 1.5	7.14
SULT2A1*6	61.4 ± 3.6	9.6 ± 3.6	6.37
SULT2A1*7	81.6 ± 5.0	11.1 ± 1.4	7.38
SULT2A1*8	73.5 ± 3.4	10.8 ± 1.1	6.79
SULT2A1*9	73.3 ± 5.2	10.0 ± 1.5	7.34
SULT2A1*10	62.8 ± 5.3	8.7 ± 1.4	7.22
Tibolone as Substrate
SULT2A1*1	3.6 ± 0.2	13.0 ± 1.7	0.28
SULT2A1*2	3.6 ± 0.1	8.7 ± 0.8	0.41
SULT2A1*3	2.8 ± 0.2	8.9 ± 1.4	0.32
SULT2A1*4	5.2 ± 0.3	10.9 ± 1.5	0.47
SULT2A1*5	3.4 ± 0.2	10.1 ± 1.4	0.34
SULT2A1*6	2.9 ± 0.2	14.3 ± 2.0	0.20
SULT2A1*7	3.7 ± 0.4	12.6 ± 2.3	0.29
SULT2A1*8	2.8 ± 0.3	16.0 ± 3.0	0.18
SULT2A1*9	3.8 ± 0.4	11.0 ± 2.2	0.34
SULT2A1*10	2.3 ± 0.2	8.9 ± 1.6	0.26

Data shown represent mean ± standard deviation calculated based on the results obtained from four independent kinetic experiments.

SULT, cytosolic sulfotransferase.