Table 2.
Effect of different inoculation and co-cultivation media on Agrobacterium-mediated transformation of Petunia hybrida
| Inoculation and co-cultivation media | Kanamycin-resistant plantlet (%) | No. of PCR- positive plantletd | No. of GUS-positive plantlete | Transformation efficiency (%)f | ||||
|---|---|---|---|---|---|---|---|---|
| Alb | LF Alc | Al | LF Al | Al | LF Al | Al | LF Al | |
| Medium 1: MS full strength | 5.00 ± 1.00d | 4.00 ± 1.60c | 2/5 | 1/4 | 0/5 | 0/4 | 0.00 ± 0.00d | 0.00 ± 0.00c |
| Medium 2: MS—(KH2PO4, NH4NO3, KNO3)a | 12.00 ± 1.90c | 9.00 ± 1.30b,c | 9/12 | 6/9 | 7/12 | 4/9 | 7.00 ± 1.80c | 4.00 ± 1.50b |
| Medium 3: MS—(KH2PO4, NH4NO3, KNO3, CaCl2) | 30.00 ± 2.00a | 25.00 ± 1.90a | 28/30 | 21/25 | 22/30 | 16/25 | 22.00 ± 2.30a | 16.00 ± 2.50a |
| Medium 4: No MS elements and vitamins only distilled water, sucrose and agar | 20.00 ± 1.95b | 13.00 ± 1.60b | 16/20 | 10/13 | 13/20 | 8/13 | 13.00 ± 2.00b | 8.00 ± 1.60b |
In each treatment, 100 leaf explants from each cultivar were inoculated and the experiment was repeated five times. The data were collected as a mean of five replications ± standard error
Various letters shows significant differences at P ≤ 0.05 (n = 5)
aThe elements inside the bracket were removed from MS medium
bAlvan cultivar
cLF Alvan cultivar
dThe number of putative transgenic plantlets with positive PCR responses/The number of putative transgenic plantlets (kanamycin resistance)
eThe number of GUS-positive plantlets was obtained from the number of kanamycin resistant plantlets
fGUS-positive plantlets were used for investigation of percentage of transformation efficiency from 100 explants