Fig. 3.

Mitochondrial aggregation enhances IR-induced cell death via amplification of ROS. a–c Cumulative cell death after irradiation of MDA-MB-231 cells: parental cells treated with siRNA targeting AMAP1, Arf6, or PRKD2 (a), cells stably transfected with mtCAT and SOD2 followed by the indicated siRNAs (b), and cells stably transfected with FKBP-MTS (MTS) and/or BICD2N-FRB* (BICD2N) followed by Rapalog treatment (c). d, e Quantification of DHE fluorescence before irradiation (d), and cumulative cell death after irradiation (e), in the presence or absence of 50 nM H₂O₂. f Activation of caspase-3 in MDA-MB-231 cells treated with the indicated siRNAs examined by western blot. g Cumulative cell death after irradiation of MDA-MB-231 cells with siRNA targeting AMAP1 or Arf6, in the presence of 50 μM Z-VAD-FMK. All graphs indicate the mean ± SEM of three independent experiments. * and ^#P < 0.05; ** and ^##P < 0.005 (two-tailed t-test, adjusted by the Holm–Sidak method). * and **, comparison to si-NC samples or non-treated cells; ^# and ^##, comparison to the corresponding samples shown in (a)