Fig. 6.

Regulation of mitochondrial trafficking via FAs is essential for cancer invasion. a Protein expression of TRAK1 and TRAK2 in different breast cancer cell lines analyzed by western blotting. β-actin was also analyzed as a control. Invasiveness (Inv) is indicated as either high (H) or low (L). b, c Protein (black bars) or mRNA (white bars) expression of TRAK1 (b) and TRAK2 (c) quantified by densitometry of the western blots (a) or qPCR, respectively. d, e Matrigel invasion of MDA-MB-231 cells transfected with siRNAs targeting TRAK1 or TRAK2 (d) or stably expressing FKBP-MTS (MTS) and/or BICD2N-FRB* (BICD2N) (e). In e, cells were treated with vehicle or AP21967 (Rapalog) before and during the assay, as indicated. f Velocity of the random migration of MDA-MB-231 cells transfected with siRNAs targeting TRAK1 or TRAK2. All the graphs indicate the mean ± SEM for three independent experiments. * and **P < 0.05 and P < 0.005 (two-tailed t-test, adjusted by the Holm–Sidak method), compared to the corresponding samples, respectively. N.S. not statistically significant