Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 11;8:10439. doi: 10.1038/s41598-018-28714-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Loss of Zic2 leads to downregulation of the NODAL pathway. (a,b) Microarray analysis of gene expression changes in the Zic2^iso/iso embryo compared to wildtype at E8.5. (a) Volcano plot. The data are plotted as log fold change (relative to wildtype) against adjusted p-value. (b) Heat map showing wildtype (WT; left panel) and Zic2^iso/iso (KO; right panel) embryos. Data show mean expression for three independent biological replicates of pooled embryos and are expressed as log2 values. (c) SYBR Green based qRT-PCR analysis of genes identified in the microarray screen, and known NODAL pathway genes. Expression values shown are normalised to Gapdh. Three independent biological replicates of pooled embryos (0–4 somites) were performed for each condition and these are plotted as individual data points (blue circles indicate wildtype; orange triangles indicate Zic2^iso/iso). Asterisks above each column indicate result of a one tailed t-test of samples with unequal variance testing the null hypothesis that loss of Zic2 has no effect on gene expression: ***p < 0.0005; **p < 0.005; *p < 0.05, NS = not significant. (d) Taqman qRT-PCR analysis of Pitx2c expression at three different ages (2–4 somites, 4–6 somites and 12–25 somites). Three independent biological replicates of pooled embryos were performed for each condition. Labels as per panel c.