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. 2018 Jul 11;8:10439. doi: 10.1038/s41598-018-28714-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Disrupted left-side specification in the Zic2 mutant. (a,b; d–g; i–l; o–r; t,u) in situ hybridisation. Genotype and age of embryos (expressed as somite number) are indicated. All images are oriented with the left side on the right of the photo and show the ventral surface of the embryo. Arrows indicate LPM, arrowheads: node. t and u are composite images. (c,h,m,n,s) Charts summarise the results of in situ experiments illustrating the relationship of expression pattern and expression level to embryo age and genotype. Each dot represents an individual embryo, blue dots are control embryos (+/+ and +/iso), red are mutants (iso/iso). A dark colour indicates strong expression while a lighter colour indicates weak or absent expression. Data are plotted as expression pattern (one of 4 categories shown on the x axis) against embryo age (expressed as somite number: 1 s, 2 s etc., y axis). HF = headfold stage. L = left, R = right.