Table 2.
Neural tube, visceral and cardiovascular defects identified in mouse Zic2 mutants.
| iso | TALEN | Trans-hets | ||||||
|---|---|---|---|---|---|---|---|---|
| iso/iso | A5/A5 | A10/A10 & A17/A17 | A19/A19 | iso/A5 | iso/A8 | |||
| Neural Tube Defects | Exencephaly | 17/17 | 10/10 | 3/3 | 3/3 | 2/2 | 2/2 | |
| Holoprosencephaly | Alobar | 13/16 | 5/7 | 3/3 | 2/3 | 2/2 | 2/2 | |
| Semilobar | 3/16 | 2/7 | 0/3 | 1/3 | 0/2 | 0/2 | ||
| Lobar | 0/16 | 0/7 | 0/3 | 0/3 | 0/2 | 0/2 | ||
| Eyes | Cyclopia | 8/17 | 3/7 | 1/3 | 2/3 | 1/2 | 2/2 | |
| Hypotelorism | 6/17 | 3/7 | 1/3 | 0/3 | 0/2 | 0/2 | ||
| Absent | 3/17 | 0/7 | 1/3 | 1/3 | 1/2 | 0/2 | ||
| Spina bifida | 17/17 | 10/10 | 3/3 | 3/3 | 2/2 | 2/2 | ||
| Curly tail | 17/17 | 10/10 | 3/3 | 3/3 | 2/2 | 2/2 | ||
| Viscera | Lungs | Right Isomerism | 13/17 | 6/10 | 2/3 | 3/3 | 1/2 | 2/2 |
| Left Isomerism | 0/17 | 1/10 | 0/3 | 0/3 | 0/2 | 0/2 | ||
| Reversed | 0/17 | 0/10 | 0/3 | 0/3 | 0/2 | 0/2 | ||
| Normal | 4/17 | 3/10 | 1/3 | 0/3 | 1/2 | 0/2 | ||
| Spleen | Reduced or absent | 1/6 | 4/7 | 0/2 | 0/0 | 0/1 | 0/0 | |
| Duplicated | 0/6 | 0/7 | 0/2 | 0/0 | 0/1 | 0/0 | ||
| Right sided | 2/6 | 0/7 | 0/2 | 0/0 | 0/1 | 0/0 | ||
| Heart | Dextrocardia | 4/16 | 4/10 | 0/3 | 2/3 | 0/2 | 1/2 | |
| Mesocardia | 7/16 | 2/10 | 0/3 | 1/3 | 2/2 | 1/2 | ||
| Stomach | Right sided | 7/17 | 2/10 | 0/3 | 0/3 | 0/2 | 2/2 | |
| Pancreas | Right sided | 5/14 | 2/7 | 0/3 | 0/2 | 0/2 | 0/0 | |
| Atria & Veins | Bilateral systemic venous sinus | 10/15 | 4/10 | 1/3 | 3/3 | 1/2 | 2/2 | |
| Left sided Inferior vena cava | 5/16 | 3/10 | 0/3 | 1/3 | 0/2 | 2/2 | ||
| Hepatic vein drainage direct to atria | 2/16 | 3/10 | 0/3 | 3/3 | 0/2 | 0/2 | ||
| Atrial septal defect | 8/11 | 5/10 | 1/3 | 2/2 | 1/2 | 2/2 | ||
| Ventricles & Arteries | Abnormal ventricular topology | 6/13 | 6/10 | 0/3 | 1/2 | 1/2 | 2/2 | |
| Great artery transposition | 0/13 | 0/10 | 1/3 | 0/3 | 0/2 | 0/2 | ||
| Double outlet right ventricle | 5/13 | 5/9 | 0/3 | 2/3 | 0/2 | 2/2 | ||
| Ventricular septal defect | 11/15 | 7/10 | 0/3 | 1/1 | 2/2 | 2/2 | ||
| Rightward looped aortic arch | 5/17 | 3/10 | 1/3 | 2/3 | 0/2 | 0/2 | ||
A summary of the phenotypes observed by MRI and µCT imaging in all Zic2 mutants examined including both the original iso line and TALEN-generated lines. All embryos are homozygous mutants, except for those labelled “trans-hets” which carry one iso allele and one TALEN allele as a test of complementarity. For each phenotype described, the first number indicates the number of embryos observed with that phenotype, while the second indicates the number examined. The latter number differs for different phenotypes because it was not possible to assess every phenotype in every embryo due to limitations of imaging.