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. 2018 Jul 11;8:10446. doi: 10.1038/s41598-018-28842-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CY5-cCATH-2 penetrates a P. aeruginosa biofilm. Representative image of a P. aeruginosa biofilms treated with 20 μM CY5-cCATH2 for 1 h at 37 °C. After treatment, the biofilm was stained with SYTO 9 (green). Bound CY5-cCATH2 appears blue. Images were acquired by CLSM using an ACS APO 63.0 × 1.30 oil objective lens. A representative orthogonal view of Z-stacks of CY5-cCATH-2-treated biofilms is shown.