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. 2018 Jul 11;8:10426. doi: 10.1038/s41598-018-28745-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LOX-PP protein purification and antibody production: (a) SDS-PAGE of purified LOX-PP using Ni-NTA agarose (Lane-M: Mw Marker, lane-1: Crude, lane-2: Unbound, lane-3 to 8: washes 1 to 6, lane-9 to 13: Elution −1 to 5). (b) Western blot for His-tag and LOX-PP in purified protein (M - Mw marker, lane-1: purified protein stained with coomassie stain). The corresponding full length blots are represented in Supplementary Fig. 21. (c) Western blot of purified protein post His-tag cleavage using factor Xa protease (Lane-1: His-tag uncleaved, Lane-2: His-tag cleaved. The corresponding full length blots are represented in Supplementary Figs 22 and 23. (d) Mass spectrum of the purified LOX-PP and its protein coverage map. (e) Direct ELISA for LOX-PP with purified antibody showing the affinity of the raised antibody with purified LOX-PP protein. (f) SDS-PAGE of purified LOX-PP antibody using two different volumes (Lane-1: 2.5 μl, Lane-2: 5.0 μl) and stained with coomassie stain to show heavy chain (HC) at 55 kDa and light chain (LC) at 25 kDa.